Dwell-time distributions, waiting-time distributions, or distributions of pause durations are widely reported in single molecule biophysical experiments. Simplified models have enabled kinetic analyses of many systems, but they may misrepresent the data if reverse and branching kinetic pathways are present. We have developed a novel computational method that overcomes these limitations and can handle complex kinetic schemes. Furthermore this method can be used to globally fit data under different conditions with a single kinetic scheme. This method was originally developed for the analysis of single molecule dwell-time distributions of myosin V under different concentrations and optical trap forces.

Reference:

Liao, J.-C., Spudich, J.A., Parker, D., Delp, S.L. (2007) Extending the absorbing boundary method to fit dwell-time distributions of molecular motors with complex kinetic pathways. Proceedings of the National Academy of Sciences, USA, 104, 3171-3716.
http://www.pnas.org/cgi/content/abstract/104/9/3171